Seminars & Webinars
78 items
Other
Optimizing AAV and LV Purification and Analytics: Accelerated High-Quality Processes using CIM® Monoliths and PATfix®
Explore Hana Jug's presentation from BPI East 2025 on optimizing AAV and LV purification using CIM® Monoliths and PATfix. Learn how these tools ensure high purity, effective impurity removal, and comprehensive sample analysis, enhancing efficiency and quality in biopharmaceutical production.
Orthogonal PAT Analytics – Essential to Enable Robust Production of Viral Vectors and LNPs
Explore the challenges and innovative solutions in lipid nanoparticle (LNP) drug delivery, focusing on targeted LNPs (tLNPs) and the use of PATfix LNP Switcher for robust analytics and process development.
Advancing Nucleic Acid Therapeutics: Scalable Strategies for pDNA, mRNA, and LNP Purification Supported by Rapid At-Line Analytics
Explore Hana Jug's presentation from BPI East 2025 on optimizing AAV and LV purification using CIM® Monoliths and PATfix. Learn how these tools ensure high purity, effective impurity removal, and comprehensive sample analysis, enhancing efficiency and quality in biopharmaceutical production.
Next-Gen LNP Pharmaceuticals Analytics: Intact Chromatography for Comprehensive Characterization
Discover innovative chromatography techniques for LNP pharmaceuticals, focusing on comprehensive characterization and process optimization.
A Novel Approach for dsRNA Removal Using Oligo dT Affinity Chromatography by Targeting Hydrogen Bond
Explore Hana Jug's presentation from BPI East 2025 on optimizing AAV and LV purification using CIM® Monoliths and PATfix. Learn how these tools ensure high purity, effective impurity removal, and comprehensive sample analysis, enhancing efficiency and quality in biopharmaceutical production.
Comprehensive Evaluation of RNA Integrity, Nanoparticle Properties, and Lipid Composition in RNA LNP
Explore Hana Jug's presentation from BPI East 2025 on optimizing AAV and LV purification using CIM® Monoliths and PATfix. Learn how these tools ensure high purity, effective impurity removal, and comprehensive sample analysis, enhancing efficiency and quality in biopharmaceutical production.
Achieving Scalable, High-Yield Plasmid DNA Purification Processes
Explore our recorded webinar on overcoming bottlenecks in plasmid (p)DNA downstream processing. Learn optimization strategies for lysis to sterile filtration, achieving high yield, purity, and compliance. Discover case studies showcasing integrated pDNA purification with real-time process monitoring and at-line analytics for enhanced performance and quality assurance.
Virtual Event: Addressing Challenges in pDNA, mRNA, and LNP Development, CMC, and Production
Access the recordings of our virtual event addressing challenges in pDNA, mRNA, and LNP development, CMC, and production. Gain insights from leading experts like Dan Peer and Jacek Lubelski on gene expression modulation, RNA delivery, and LNP manufacturing. Explore scalable purification workflows, innovative approaches to gene editing, and advanced analytical techniques for therapeutic applications. Enhance your understanding of nucleic acid delivery and LNP complexity with practical solutions for overcoming CMC hurdles.
Webinar with Refeyn: AAV6 Capsid Optimization with CIM SO3 Plates & Mass Photometry
Speakers: Hena Divanovic, Application Scientist at Refeyn Marko Narobe, Product Manager for High Throughput Solutions at Sartorius BIA SeparationsWatch the webinar to learn how to: The use of CIM SO3...
Method Optimization & High Throughput Purification for mRNA and rAAV
Speaker: Marko Narobe, Sartorius BIA SeparationsAbstract: The downstream processing of virus particles, vesicles, RNAs, plasmids and other forms of DNA, contains multiple interdependent steps, each requiring optimization for best results....
The Importance of Viral Clearance and Characterization in Gene Therapy Products
Speakers: Ya-Chen Chang, Regenxbio Akunna Iheanacho, Texcell Katelyn Pritchard, Texcell Maja Leskovec, Sartorius BIA Separations Maja Leskovec, Head of Process Development Viruses at Sartorius BIA Separations, presented "High Resolution Chromatography...
Advancing EV Research: Analytical Chromatography Solutions for Efficient and Comprehensive Characterization of EV Preparations With Varying Complexity
Speakers: Darja Božič, Project Manager, Sartorius BIA SeparationsAbstract: The field of extracellular vesicles (EV) has been increasingly empowered in recent decades, with significant advances in sophisticated analytical tools and the...
Optimizing Plasmid DNA Purification | Webinar with VGXI
Speakers: Christy Franco, Director, Commercial Operations at VGXI Jasmina Puc, Product Manager for Nucleic Acids at Sartorius BIA SeparationsWatch the webinar to learn how to: Enhance robustness and improve contaminant...
Enhancing Manufacturing Efficiency and Quality Through Optimized Lysis in Plasmid DNA Extraction
Speakers: Robin Pelizzari, Scientist pDNA small scale DSP Development, mRNA Center of Excellence at Sanofi Vaccine, France Matevž Korenč, Product Manager at Sartorius BIA Separations, SloveniaWatch the webinar to learn...
Accelerate Your AAV Process Development With a New Wave Analytical Tool
Speaker: Andreja Gramc Livk, Sartorius BIA SeparationsAbstract: Adeno-associated viruses (AAVs) are widely used vectors for gene therapies mainly due to their favorable safety profile and high transduction efficiency to various...
AAV Capsid Analysis and Quantification With Chromatography and Ultracentrifugation-Based Techniques
Speakers: Andreja Gramc Livk, Head of Process Analytics, Sartorius BIA Separations Sebastijan Peljhan, Head of Basic Research, Sartorius BIA SeparationsAbstract: This webinar will explore empty-to-full AAV capsid ratio determination using...
In-Line Lysis System: The Easiest Alternative to Batch Alkaline Lysis
Speaker: Matevz Korenc, Product Manager, Sartorius BIA SeparationsAbout the Speaker After receiving a PhD in Biochemistry, Matevz Korenc continued as a team leader in Knauf insulation, where he managed the...
Advancing LNP Characterization for Drug Delivery: NeoVac
Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Dan Peer, Chairman, NeoVac Tristan Kovačič, Scientist, Process Analytics Development, Sartorius BIA Separations Abstract: Lipid nanoparticles (LNPs) are cell-specific delivery...
Optimizing LNP Formulations: Leveraging Monolithic Columns for Precise Insight and Improvement Strategies
Watch this webinar to learn about: How to capitalize on the advantages of scalable methods for separation of different biomacromolecules Understand the benefits of highly tunable chromatographic methods Discover how...
FastFacts: PATfix pDNA Platform for Monitoring pDNA Purification Process
Watch the video or read the poster to learn: The importance of pDNA as an enabling product in the production of mRNA, AAV, and other therapeutic vectors How the PATfix™...
Optimizing Lentiviral Purification: Enhancing Chromatography With Monoliths
Speaker: Luka Bevc, Manager Product|Application Area, Sartorius BIA SeparationsAbstract: Lentiviral vectors (LVVs) can effectively deliver up to 10 kb of target genes and can infect non-dividing cells, making them a...
Quality by Design in mRNA Manufacturing: At-Line Monitoring Coupled With DOE for Higher IVT Yield and Efficiency of Purification
Speaker: Rok Sekirnik, Head of Process Development for mRNA and pDNA, Sartorius BIA SeparationsAbstract: Due to the catalytic, multicomponent nature of the IVT reaction, optimization is a multi-factorial problem, ideally...
Enhancing mRNA Production with Advanced Analytics: WACKER Biotech
Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Michael Daume,Group leader, Downstream Process Development for Nucleic Acids, WACKER BiotechAbstract: Production of mRNA involves complex IVT mixtures, including target...
Accelerating AAV Process Development: NCSU BTEC
Speakers: Blaž Bakalar, Product Manager, PATfix® Platform, Sartorius BIA Separations Shriarjun Shastry (Arjun), Scientist II, Biomanufacturing Training and Education Center (NCSU BTEC)Abstract: Adeno-associated viruses (AAVs) are widely used vectors for...
Implementation of an Improved Platform for Downstream Processing of Plasmid DNA
Speakers: Justina Martinkiene, Scientist, Purification Sciences, AstraZeneca Klemen Božič, Process Development Scientist, Sartorius BIA SeparationsAbstract: Plasmid DNA (pDNA) represents the backbone of most cell and gene therapy production workflows. As...
Comparability of Five Alternative AAV9 Downstream Processes from Ultracentrifugation to Chromatography
Speakers: Aleš Štrancar, Executive Managing Director, Sartorius BIA Separations Joe Balleydier, Senior Vice President of Research and Development, Advanced Medicine Partners Mike Hatfield, Senior Director of Research and Development, Advanced...
Improving the Safety of Your LNP-mRNA Therapeutics and Vaccines by Utilizing Monolithic Chromatography
Speakers: Andreja Gramc Livk, Head of Process Analytics Tristan Kovačič, ScientistLipid nanoparticle (LNP) based therapeutics and vaccines have been at the forefront of the non-viral delivery since SARS-CoV-2 vaccines rolled...
Process is the Product: A New Analytical Tool for AAV Production Optimization and Monitoring
Speakers: Blaž Bakalar, Product Manager PATfix® Platform, Sartorius BIA Separations Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA SeparationsAbstract: Chromatographic analysis of complex AAV presents challenges due to...
Mastering Monolith Chromatography with DOE Strategies for Lentiviral Vector and Exosome Purification
Speakers: Maja Leskovec, Head of Process Development Viruses Luka Bevc, Scientist Andrej Raspor, ScientistAbstract: Join us for an insightful webinar on the latest advancements in monolith chromatography for lentiviral vector...
Fast & Efficient rAAV Downstream Process Optimisation With CIM Monolithic 96-Well Plates
Speaker: Mojca Tajnik Sbaizero, Project Manager, Sartorius BIA SeparationsAbstract: rAAV downstream process using monoliths contains a series of steps that are mutually dependent. Therefore each step should be optimized for...
Fast In-Process Analytics to Enable Robust mRNA Manufacturing
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Typical mRNA production process involves four key steps: 1) plasmid DNA (pDNA) expression in E.coli, linearization and pDNA purification, 2) in-vitro transcription...
From Process Development to Manufacturing: How to Successfully Optimize and Scale up the AAV Enrichment Step on Novel CIMmultus QA HR Line
Watch the presentation to learn about: Chromatographic purification of AAV Development of the enrichment step Improving resolution and reproducibility with the CIMmultus HR line Successful scale up Speaker: Rok...
Enabling Tools for Purification and Understanding Complex Biomolecules Structure
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA Separations
Exosomes: New Tools for Industrial Purification and Process Monitoring
Speaker: Pete Gagnon, Sartorius BIA Separations
Cornerstone Technology for Purification of Clinical-Quality AAV
Cornerstone Technology for Purification of Clinical-Quality AAV.mpeg 39,75 MB
Analytical Methods: The Path to More Efficient Processes & Safer Gene Therapy Products
Speakers: Aleš Štrancar, Managing Director, Sartorius BIA Separations
A Cornerstone Platform for Industrial Purification of Exosomes
Speakers: Pete Gagnon, Sartorius BIA Separations
CIMmultus Monoliths – Tailored to Accelerate Process Development and Address Large Scale Manufacturing of ATMPs and Vaccines
Speakers: Ingo Nagler
CIMmultus Monoliths – Tailored to Accelerate Process Development and Address Large Scale Manufacturing of ATMPs like AAV, pDNA, mRNA or Exosomes
Speaker: Ingo Nagler
Purification of Single-Stranded mRNA: The Toolbox for the Next Generation
Abstract: Messenger RNA is poised to become a major contributor in the fields of gene therapy and vaccines. Making this a practical reality requires purification technology that accommodates its unique...
Chromatin (DNA) Removal From Harvest Before an AAV Capture Step Greatly Improves Robustness, Purity, and Yield of the Overall Downstream Process
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low toxicity, good overall safety...
Platform Purification of Clinical Quality Bacteriophage
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Many of the most compelling applications of bacteriophages involve human therapy, some pertinent to gene therapy, others to antibiotic replacement. These applications...
High Yield mRNA Production Process from E.Coli to Highly Pure mRNA
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Established processes for pDNA manufacturing include purification steps designed to enrich the supercoiled (sc) isoform. Homogeneous supercoiled isoform improves cell transfection efficiency...
Ways to Improve Empty, Full and Damaged Capsids Separation
This webinar features: Buffer optimisation and proper column ligand selection (CIM QA, CIM PrimaS or new CIM EF) allows for >90% full capsid production Introduction of Specimen allows for production...
Chromatographic Tools for High-Yielding mRNA Production Process & Integrated Production Process of mRNA From E. Coli to Highly Purified mRNA
Our new Head of Process Development for mRNA and pDNA purification, Rok Sekirnik, presented our Chromatographic tools for high-yielding mRNA production process as workshop. Our CEO, Ales Štrancar presented our...
Efficient, Robust and Scalable Purification of Covid Vaccines Based on pDNA, mRNA, Adeno and VLP Technologies
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low toxicity, good overall safety...
In-Process Analytics of AAV Vector Capsid Production
Speaker: Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA SeparationsAbstract: In-process analytics of vector capsid production is a critical optimization target in development of AAV-based gene therapy products....
Unlock the Potential of the mRNA Vaccine Platform with Integrated Production Processes from E. coli to Highly Purified mRNA
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: A new purification approach starting from E.Coli all through to mRNA production is presented here. This new approach integrates a pDNA linearisation...
From E. coli to mRNA Drug Substance – Development of Analytical and Purification tools for a scalable mRNA production process
Speaker: Rok Sekirnik, Sartorius BIA SeparationsAbstract: This talk presents case studies of leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA production....
Acceleration of AAV Manufacturing Process Development by Using Fast USP and DSP At-Line Biochromatography Analytics
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low toxicity, good overall safety...
All-in-one PATfix Analytical Platform
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA SeparationsAbstract: Global demand for pDNA production is at an all time high, due to increased need from Gene Therapy ramp-up. pDNA, as an...
FastFacts: Adenovirus Vector Manufacturing Platform Using CIMmultus QA Assures the Supply of Safe Vaccines
Watch the video or read the poster to learn: Why downstream processing remains one of the main bottlenecks in adenoviral vector manufacturing How an adenoviral vector purification platform using CIMmultus™ QA as...
Optimization of the AAV Expression Using PATfix Analytical Platform
Speaker: Ivana Petrović Koshmak, Head of Upstream Process Development, Sartorius BIA SeparationsAbstract: Adeno associated virus (AAV) is the leading vector in the field of gene therapy because of its low...
FastFacts: mRNA Manufacturing Platform Using CIMmultus® and CIMac Technology for High Recovery of Stable mRNA Drug Substance with Rapid HPLC Analytics
Watch the video or read the poster to learn: Non-affinity chromatography with PrimaS provides purification for IVT processes with co-transcriptional polyadenylation The Sartorius mRNA purification toolbox provides a scalable chromatographic...
FastFacts: Fast Chromatography of AAV – Purification and Analytics
Watch the video or read the poster to learn: Importance of sample pretreatment before first chromatographic step Rapid downstream process of AAV with emphasis on chromatographic step of CIMmultus SO3...
Speeding mRNA Process Development & Securing Robust Manufacturing by Using Fast In-Process Analytics
Speaker: Aleš Štrancar, Managing Director, Sartorius BIA Separations
Optimize Your mRNA Process from pDNA Template to LNP Encapsulation with a Single Analytical Platform
Speaker: Blaž Bakalar, Product Manager PATfix platform, Sartorius BIA SeparationsAbstract: mRNA-based therapeutics represent a promising new modality for therapies and vaccines. However, industry-wide process understanding of the CQA and CPP...
Optimize Your Process From pDNA Template to LNP Encapsulation With the Help of a Single Analytical Platform
Abstract: PATfix mRNA analytical platform is a perfect tool for process developers looking to optimize their mRNA production and purification Three orthogonal analytical methods based on mix-mode, affinity and reverse...
AAV Manufacturing Process Development Using Fast USP and DSP Biochromatography Analytics
Key learning points: One of the key challenges in manufacturing viral vectors is to increase the ratio between empty and full capsids. When the expression in the cell line results...
mRNA: Removing Bottlenecks in Process Development and Manufacturing
Speaker: Rok Sekirnik, Head of Process development for mRNA|pDNA, Sartorius BIA SeparationsAbstract: In vitro-transcribed messenger RNA-based therapeutics represent a relatively novel and highly efficient class of drugs. Current clinical efforts...
Robust Manufacturing of Purer AAV Gene Therapy Vectors
Attend this webinar to learn how to achieve: 10% improved process recovery using the strong cation exchange instead of affinity column up to 2 fold shorter processing time using SO3...
Increasing Productivity of IVT Reaction Using At-Line Monitoring by HPLC Chromatography
In this webinar, we will discuss an HPLC-based analytical method using a multimodal ligand based on anion exchange/hydrogen-bonding ligand (PrimaS) to monitor the IVT reaction, which allows for simultaneous quantification...
Overview of Downstream Process of AAV Purification Using Monoliths and Rapid Evaluation of AAV Empty-Full Ratio with the PATfix System
Key learning points: Rapid downstream process of AAV using monoliths, with emphasis on PATfix analytical tools Orthogonal analytics are paramount for AAV characterization Integration using PATfix enables fast evaluation of...
Meeting Demand for Clinical Production of mRNA
Watch this webinar to learn about: How to ensure consistent and robust IVT production of mRNA How to achieve high yield and high purity in mRNA purification processes Chromatography solutions...
Increasing Productivity of IVT Reaction Using At-Line Monitoring by HPLC Chromatography
Watch this webinar to learn about: Benefits of implementing HPLC-based analytics in mRNA production workflow Principles of monolith chromatography and its use for mRNA analytics and purification Principles of IVT...
PATfix Analytical Platform for Monitoring mRNA Production Process
In this webinar, will discuss a robust analytical tool PATfix platform, and its specific methods for pDNA and mRNA analytics that enable efficient monitoring of key upstream and downstream process...
Chromatography in mRNA Production: From Analytics to Purification
Speaker: Rok Sekirnik, Head Process Development for mRNA and pDNA, Sartorius BIA SeparationsAbstract: The recently demonstrated efficacy of mRNA-based Covid-19 vaccines has shown the promise of this therapeutic format, but...
Cost Efficient Manufacturing of Purer AAV Gene Therapy Vectors
10% improved process recovery using the strong cation exchange instead of affinity column Up to 2 fold shorter processing time using SO3 instead of affinity column Less damaged capsids using...
Improved Capsid Purification in AAV Manufacturing
Speaker: Aleš Štrancar, Sartorius BIA SeparationsAbstract: Adeno-associated virus (AAV) capsids are an effective mechanism for delivering gene therapies intended for therapeutic use. A critical production step is the separation of...
New Methods to Separate Empty, Partial, Full and Heavy AAV Capsids
Learn how to achieve: Five times better resolution of empty/full capsids Large scale removal of empty, partial and heavy AAV capsids Batch-to-batch and scale-to-scale reproducibility within +/- 3% Speaker: Aleš...
New Empty|Full AAV Method Supported With Novel CIMmultus HR Columns to Enable Robust Manufacturing of AAV Products
Speaker: Aleš Štrancar, Sartorius BIA SeparationsAbstract: Separation of empty and full AAV capsids with almost same chromatographic characteristic request very high-resolution chromatography media and scale-to scale reproducibility within just few...
Chromatographic Tools for Isolation of Extracellular Vesicles and Process Monitoring Analytics
Speaker: Katja Vrabec, Project Manager, Sartorius BIA SeparationsAbstract: The development of fast and efficient processes for the isolation of extracellular vesicles (EVs) depends on the availability of chromatography media that...
Chromatography in mRNA Production: From Analytics to Purification
Speaker: Rok Sekirnik, Head Process Development for mRNA and pDNA, Sartorius BIA SeparationsAbstract: The recently demonstrated efficacy of mRNA-based Covid-19 vaccines has shown the promise of this therapeutic format, but...
Chromatographic Modalities, Buffer Selection and Other Considerations for Enrichment of AAV Product
Speaker: Rok Žigon, Head of Product-Application Area (AAV|Adeno), Process Development VirusesAbstract: Adeno-associated virus (AAV) is go-to vector for gene therapy treatments. In each AAV downstream process, one of the key...
Chromatography in mRNA Production From Analytics to Purification
Speaker: Rok Sekirnik, Sartorius BIA SeparationsAbstract: This talk presents case studies of leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA production. It describes...
An Innovative Analytical Platform for Evaluating Vital LNP Characteristics
Speaker: Nejc Pavlin, Project Manager, Sartorius BIA SeparationsAbstract: Lipid nanoparticles (LNPs) have emerged as versatile drug delivery systems capable of encapsulating various payloads, including different nucleic acids. LNPs offer numerous...
Maximizing mRNA Production: At-line Process Monitoring for Improved Purity and Yield
Speaker: Rok Sekirnik, Sartorius BIA SeparationsAbstract: The talk will present case studies of leveraging the power of at-line HPLC monitoring for process development to increase purity and yield of mRNA...
Scalable Chromatographic Strategies for mRNA and saRNA Purification From IVT
Watch this webinar to learn about: Ins-and-outs of affinity chromatography for mRNA purification Challenges faced with purification of self-amplifying RNA Alternatives to affinity chromatography and their use in purification of...